Open AccessMatrix Metalloproteinase MMP-12 Promotes Macrophage Transmigration Across Intestinal Epithelial Tight Junctions and Increases Severity of Experimental Colitis
Author(s) -
Meghali Nighot,
Ashwinkumar Subramenium Ganapathy,
Kushal Saha,
Eric Suchanec,
Eliseo F. Castillo,
Alyssa D. Gregory,
Steven D. Shapiro,
Thomas Ma,
Prashant K. Nighot
Publication year - 2021
Publication title -
journal of crohn's and colitis
Language(s) - English
Resource type - Journals
eISSN - 1876-4479
pISSN - 1873-9946
DOI - 10.1093/ecco-jcc/jjab064
Subject(s) - barrier function , matrix metalloproteinase , basement membrane , inflammation , tight junction , colitis , laminin , intestinal permeability , macrophage , ussing chamber , ex vivo , immunology , in vivo , extracellular matrix , inflammatory bowel disease , chemistry , biology , microbiology and biotechnology , pathology , epithelium , medicine , in vitro , biochemistry , disease
Matrix metalloproteinases [MMPs] play an important role in extracellular matrix regulation during cell growth and wound healing. Increased expression of MMP-12 [human macrophage elastase] has been reported in inflammatory bowel disease [IBD] which is characterised by the loss of epithelial tight junction [TJ] barrier function and an excessive inflammatory response. The aim of this study was to investigate the role of MMP-12 in intestinal TJ barrier function and inflammation.