Open AccessLamprey buccal gland secretory protein-2 (BGSP-2) inhibits human T lymphocyte proliferation
Author(s) -
Jing Sun,
Shui-Yan Yu,
Xue Zhao,
Cenjie Liu,
Yu Wu,
Xin Liu,
Qingwei Li
Publication year - 2010
Publication title -
current zoology/environmental epigenetics/current zoology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.971
H-Index - 38
eISSN - 2058-5888
pISSN - 1674-5507
DOI - 10.1093/czoolo/56.2.252
Subject(s) - lamprey , biology , secretion , buccal administration , complementary dna , microbiology and biotechnology , gene , endocrinology , biochemistry , pharmacology , fishery
Lamprey is a representative of the agnathans, the most ancient class of vertebrates. Parasitic lampreys secrete anticoagulant from their buccal glands and prevent blood coagulation of host fishes. We identified a buccal gland secretory protein-2 (BGSP-2) from a buccal gland cDNA library of Lampetra japonica. The full-length BGSP-2 gene was cloned and the recombinant BGSP-2 protein was generated. The role of BGSP-2 on lymphocyte proliferation was studied by examining its effects on human T lymphocytes. We found that lamprey BGSP-2 was able to effectively block the proliferation of T cells in vitro by inducing G1/S cell cycle arrest. Furthermore, it inhibited the proliferation of human T lymphocytes stimulated by phytohemagglutinin (PHA) at a minimum concentration of 0.μg/ml. Our data suggest that lamprey BGSP-2 is able to block the mitosis of human T lymphocytes at the G1/S point, and has the potential of anti-proliferative effect on PHA-activated T lymphocytes.