Open AccessLight sheet fluorescence microscopy as a new method for unbiased three-dimensional analysis of vascular injury
Author(s) -
Nicholas Buglak,
Jennifer L. Lucitti,
Pablo Ariel,
Sophie Maiocchi,
Francis J. Miller,
Edward Moreira Bahnson
Publication year - 2020
Publication title -
cardiovascular research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.774
H-Index - 219
eISSN - 1755-3245
pISSN - 0008-6363
DOI - 10.1093/cvr/cvaa037
Subject(s) - medicine , carotid arteries , biomedical engineering , radiology , pathology , surgery
Assessment of preclinical models of vascular disease is paramount in the successful translation of novel treatments. The results of these models have traditionally relied on two-dimensional (2D) histological methodologies. Light sheet fluorescence microscopy (LSFM) is an imaging platform that allows for three-dimensional (3D) visualization of whole organs and tissues. In this study, we describe an improved methodological approach utilizing LSFM for imaging of preclinical vascular injury models while minimizing analysis bias.