Open AccessMolecular Approach to Diagnosis of Cardiovascular Implantable Electronic Device Infection
Author(s) -
Zerelda Esquer Garrigos,
M. Rizwan Sohail,
Kerryl E. GreenwoodQuaintance,
Scott A. Cunningham,
Priya Vijayvargiya,
Madiha Fida,
Paul A. Friedman,
Jayawant N. Mandrekar,
Daniel C. DeSimone,
Larry M. Baddour,
Robin Patel
Publication year - 2019
Publication title -
clinical infectious diseases/clinical infectious diseases (online. university of chicago. press)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1093/cid/ciz266
Subject(s) - 16s ribosomal rna , polymerase chain reaction , ribosomal rna , medicine , microbiological culture , microbiology and biotechnology , bacteria , biology , gene , genetics
Sonicate fluid (SF), a solution derived from vortexing and sonication of explanted cardiovascular implantable electronic devices (CIEDs), is a higher-yield specimen compared with swabs or tissues for culture-based detection of microorganisms associated with CIED infection. Despite this, SF culture fails to identify a causative organism in ~50% of cases. We aimed to evaluate the diagnostic performance of 16S ribosomal RNA gene (rRNA) polymerase chain reaction (PCR)/sequencing of SF and compare it with that of SF culture.