Open AccessBuilding the Framework for Standardized Clinical Laboratory Reporting of Next-generation Sequencing Data for Resistance-associated Mutations in Mycobacterium tuberculosis Complex
Author(s) -
Jeffrey A. Tornheim,
Angela M. Starks,
Timothy C. Rodwell,
Jennifer Gardy,
Tim Walker,
Daniela María Cirillo,
Lakshmi Jayashankar,
Paolo Miotto,
Matteo Zignol,
Marco Schito
Publication year - 2019
Publication title -
clinical infectious diseases/clinical infectious diseases (online. university of chicago. press)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1093/cid/ciz219
Subject(s) - medicine , mycobacterium tuberculosis , tuberculosis , terminology , mycobacterium tuberculosis complex , clinical microbiology , dna sequencing , infectious disease (medical specialty) , computational biology , intensive care medicine , data science , disease , biology , pathology , genetics , computer science , gene , microbiology and biotechnology , philosophy , linguistics
Tuberculosis is the primary infectious disease killer worldwide, with a growing threat from multidrug-resistant cases. Unfortunately, classic growth-based phenotypic drug susceptibility testing (DST) remains difficult, costly, and time consuming, while current rapid molecular testing options are limited by the diversity of antimicrobial-resistant genotypes that can be detected at once. Next-generation sequencing (NGS) offers the opportunity for rapid, comprehensive DST without the time or cost burden of phenotypic tests and can provide useful information for global surveillance. As access to NGS expands, it will be important to ensure that results are communicated clearly, consistent, comparable between laboratories, and associated with clear guidance on clinical interpretation of results. In this viewpoint article, we summarize 2 expert workshops regarding a standardized report format, focusing on relevant variables, terminology, and required minimal elements for clinical and laboratory reports with a proposed standardized template for clinical reporting NGS results for Mycobacterium tuberculosis.