Open AccessResistance-Conferring Mutations on Whole-Genome Sequencing of Fluoroquinolone-resistant and -Susceptible Mycobacterium tuberculosis Isolates: A Proposed Threshold for Identifying Resistance
Author(s) -
Fernanda Maruri,
Yan Guo,
Amondrea Blackman,
Yuri F. van der Heijden,
Peter F Rebeiro,
Timothy R. Sterling
Publication year - 2020
Publication title -
clinical infectious diseases/clinical infectious diseases (online. university of chicago. press)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1093/cid/ciaa496
Subject(s) - dna gyrase , ofloxacin , allele , allele frequency , mycobacterium tuberculosis , genetics , biology , mutation , tuberculosis , medicine , gene , antibiotics , ciprofloxacin , escherichia coli , pathology
Fluoroquinolone resistance in Mycobacterium tuberculosis (Mtb) is conferred by DNA gyrase mutations, but not all fluoroquinolone-resistant Mtb isolates have mutations detected. The optimal allele frequency threshold to identify resistance-conferring mutations by whole-genome sequencing is unknown.