Open AccessAcanR3990 qPCR: A Novel, Highly Sensitive, Bioinformatically-Informed Assay to Detect Angiostrongylus cantonensis Infections
Author(s) -
William J. Sears,
Yvonne Qvarnström,
Eric Dahlstrom,
Kirsten Snook,
Lisa Kaluna,
Vojtěch Baláž,
Barbora Fecková,
Jan Šlapeta,
David Modrý,
Susan I. Jarvi,
Thomas B. Nutman
Publication year - 2020
Publication title -
clinical infectious diseases/clinical infectious diseases (online. university of chicago. press)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1093/cid/ciaa1791
Subject(s) - angiostrongylus cantonensis , lungworm , angiostrongyliasis , biology , toxocariasis , toxocara cati , genomic dna , paratenic , primer (cosmetics) , helminths , zoology , dna , genetics , chemistry , organic chemistry
Angiostrongylus cantonensis (Ac), or the rat lungworm, is a major cause of eosinophilic meningitis. Humans are infected by ingesting the 3rd stage larvae from primary hosts, snails, and slugs, or paratenic hosts. The currently used molecular test is a qPCR assay targeting the ITS1 rDNA region (ITS1) of Ac.