Development of an Analytical Methodology for Simultaneous Determination of Vincristine and Doxorubicin in Pharmaceutical Preparations for Oncology by HPLC--UV | Zendy

António Sebastião Rodrigues | Zendy; Ana R. Lopes | Zendy; Ariadne Gonçalves de Leão | Zendy; A. Couceiro | Zendy; Ana Bela SarmentoRibeiro | Zendy; Fernando Ramos | Zendy; M.I.N. Silveira | Zendy; Catarina R. Oliveira | Zendy

Open Access

Development of an Analytical Methodology for Simultaneous Determination of Vincristine and Doxorubicin in Pharmaceutical Preparations for Oncology by HPLC--UV

Author(s) -

António Sebastião Rodrigues,

Ana R. Lopes,

Ariadne Gonçalves de Leão,

A. Couceiro,

Ana Bela SarmentoRibeiro,

Fernando Ramos,

M.I.N. Silveira,

Catarina R. Oliveira

Publication year - 2009

Publication title -

journal of chromatographic science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.362

H-Index - 56

eISSN - 1945-239X

pISSN - 0021-9665

DOI - 10.1093/chromsci/47.5.387

Subject(s) - chemistry , chromatography , doxorubicin , acetonitrile , vincristine , high performance liquid chromatography , chemotherapy , surgery , cyclophosphamide , medicine

A high-performance liquid chromatography-UV methodology (lambda=230 nm) was developed and validated for the simultaneous determination of vincristine and doxorubicin in pharmaceutical preparations used in oncology. The chromatography was carried out on a C18 column using acetonitrile 90% in water-potassium hydrogenphosphate buffer 50 mM, pH 3.2+/-0.1 (32:68, v/v) as mobile phase at a flow rate of 1.5 mL/min. The method proved to be specific, exact, and accurate, in accordance with the ICH standards, presenting linearity in the 1-5 microg/mL and 5-100 microg/mL intervals, and detection (0.19x0.51 microg/mL) and quantification (0.63x1.7 microg/mL) limits for vincristine and doxorubicin, respectively.

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