The human brain utilizes lactate via the tricarboxylic acid cycle: a 13C-labelled microdialysis and high-resolution nuclear magnetic resonance study | Zendy

Clare Gallagher | Zendy; Keri L.H. Carpenter | Zendy; Peter Grice | Zendy; Duncan J. Howe | Zendy; Andrew Mason | Zendy; Ivan Timofeev | Zendy; David Me | Zendy; Peter J. Kirkpatrick | Zendy; John D. Pickard | Zendy; Garnette R. Sutherland | Zendy; Peter J. Hutchinson | Zendy

Open Access

The human brain utilizes lactate via the tricarboxylic acid cycle: a 13C-labelled microdialysis and high-resolution nuclear magnetic resonance study

Author(s) -

Clare Gallagher,

Keri L.H. Carpenter,

Peter Grice,

Duncan J. Howe,

Andrew Mason,

Ivan Timofeev,

David Me,

Peter J. Kirkpatrick,

John D. Pickard,

Garnette R. Sutherland,

Peter J. Hutchinson

Publication year - 2009

Publication title -

brain

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.142

H-Index - 336

eISSN - 1460-2156

pISSN - 0006-8950

DOI - 10.1093/brain/awp202

Subject(s) - tricarboxylic acid , citric acid cycle , glutamine , human brain , glutamate receptor , microdialysis , metabolism , chemistry , nuclear magnetic resonance , biochemistry , biology , neuroscience , amino acid , extracellular , physics , receptor

Energy metabolism in the human brain is not fully understood. Classically, glucose is regarded as the major energy substrate. However, lactate (conventionally a product of anaerobic metabolism) has been proposed to act as an energy source, yet whether this occurs in man is not known. Here we show that the human brain can indeed utilize lactate as an energy source via the tricarboxylic acid cycle. We used a novel combination of (13)C-labelled cerebral microdialysis both to deliver (13)C substrates into the brain and recover (13)C metabolites from the brain, and high-resolution (13)C nuclear magnetic resonance. Microdialysis catheters were placed in the vicinity of focal lesions and in relatively less injured regions of brain, in patients with traumatic brain injury. Infusion with 2-(13)C-acetate or 3-(13)C-lactate produced (13)C signals for glutamine C4, C3 and C2, indicating tricarboxylic acid cycle operation followed by conversion of glutamate to glutamine. This is the first direct demonstration of brain utilization of lactate as an energy source in humans.

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