Dissecting the chromosomal composition of mutagen-induced micronuclei inBrachypodium distachyonusing multicolour FISH | Zendy

Arita Kuś | Zendy; Jolanta Kwaśniewska | Zendy; Joanna Szymanowska-Pułka | Zendy; Robert Hasterok | Zendy

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Dissecting the chromosomal composition of mutagen-induced micronuclei inBrachypodium distachyonusing multicolour FISH

Author(s) -

Arita Kuś,

Jolanta Kwaśniewska,

Joanna Szymanowska-Pułka,

Robert Hasterok

Publication year - 2018

Publication title -

annals of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.567

H-Index - 176

eISSN - 1095-8290

pISSN - 0305-7364

DOI - 10.1093/aob/mcy115

Subject(s) - biology , brachypodium distachyon , micronucleus test , mutagen , genetics , brachypodium , bacterial artificial chromosome , dna , genome , gene , chemistry , organic chemistry , toxicity

Brachypodium distachyon (Brachypodium) is a model species for temperate cereals and other economically important grasses. Its favourable cytogenetic features and advanced molecular infrastructure make it a good model for understanding the mechanisms of instability of plant genomes after mutagenic treatment. The aim of this study was to qualitatively and quantitatively assess the composition and origin of micronuclei arising from genomic fracture, and to detect possible 'hot spots' for mutagen-induced DNA breaks.

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