Genome-wide association study dissects the genetic control of plant height and branch number in response to low-phosphorus stress in Brassica napus | Zendy

Haijiang Liu | Zendy; Jingchi Wang | Zendy; Bingbing Zhang | Zendy; Xinyu Yang | Zendy; John P. Hammond | Zendy; Guangda Ding | Zendy; Sheliang Wang | Zendy; Hongmei Cai | Zendy; Chuang Wang | Zendy; Fangsen Xu | Zendy; Lei Shi | Zendy

Open Access

Genome-wide association study dissects the genetic control of plant height and branch number in response to low-phosphorus stress in Brassica napus

Author(s) -

Haijiang Liu,

Jingchi Wang,

Bingbing Zhang,

Xinyu Yang,

John P. Hammond,

Guangda Ding,

Sheliang Wang,

Hongmei Cai,

Chuang Wang,

Fangsen Xu,

Lei Shi

Publication year - 2021

Publication title -

annals of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.567

H-Index - 176

eISSN - 1095-8290

pISSN - 0305-7364

DOI - 10.1093/aob/mcab115

Subject(s) - biology , single nucleotide polymorphism , brassica , genome wide association study , haplotype , genetics , genetic association , candidate gene , allele , genetic variation , gene , genotype , horticulture

Background and Aims Oilseed rape (Brassica napus) is one of the most important oil crops worldwide. Phosphorus (P) deficiency severely decreases the plant height and branch number of B. napus. However, the genetic bases controlling plant height and branch number in B. napus under P deficiency remain largely unknown. This study aims to mine candidate genes for plant height and branch number by genome-wide association study (GWAS) and determine low-P-tolerance haplotypes. Methods An association panel of B. napus was grown in the field with a low P supply (P, 0 kg ha−1) and a sufficient P supply (P, 40 kg ha−1) across 2 years and plant height and branch number were investigated. More than five million single-nucleotide polymorphisms (SNPs) were used to conduct GWAS of plant height and branch number at two contrasting P supplies. Key Results A total of 2127 SNPs were strongly associated (P < 6·25 × 10−07) with plant height and branch number at two P supplies. There was significant correlation between phenotypic variation and the number of favourable alleles of associated loci on chromosomes A10 (chrA10_821671) and C08 (chrC08_27999846), which will contribute to breeding improvement by aggregating these SNPs. BnaA10g09290D and BnaC08g26640D were identified to be associated with chrA10_821671 and chrC08_27999846, respectively. Candidate gene association analysis and haplotype analysis showed that the inbred lines carrying ATT at BnaA10g09290Hap1 and AAT at BnaC08g26640Hap1 had greater plant height than lines carrying other haplotype alleles at low P supply. Conclusion Our results demonstrate the power of GWAS in identifying genes of interest in B. napus and provided insights into the genetic basis of plant height and branch number at low P supply in B. napus. Candidate genes and favourable haplotypes may facilitate marker-based breeding efforts aimed at improving P use efficiency in B. napus.

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