Open AccessSynaptobrevin-2 dependent regulation of single synaptic vesicle endocytosis
Author(s) -
Natalí L. Chanaday,
Ege T. Kavalali
Publication year - 2021
Publication title -
molecular biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.463
H-Index - 225
eISSN - 1939-4586
pISSN - 1059-1524
DOI - 10.1091/mbc.e21-04-0213
Subject(s) - synaptobrevin , biology , vesicle fusion , synaptic vesicle , endocytosis , bulk endocytosis , snap25 , kiss and run fusion , vesicle , microbiology and biotechnology , synaptic vesicle recycling , exocytosis , biophysics , biochemistry , receptor , membrane
Evidence from multiple systems indicates that vesicle SNARE (soluble NSF attachment receptor) proteins are involved in synaptic vesicle endocytosis, although their exact action at the level of single vesicles is unknown. Here we interrogate the role of the main synaptic vesicle SNARE mediating fusion, synaptobrevin-2 (also called VAMP2), in modulation of single synaptic vesicle retrieval. We report that in the absence of synaptobrevin-2, fast and slow modes of single synaptic vesicle retrieval are impaired, indicating a role of the SNARE machinery in coupling exocytosis to endocytosis of single synaptic vesicles. Ultrafast endocytosis was impervious to changes in the levels of synaptobrevin-2, pointing to a separate molecular mechanism underlying this type of recycling. Taken together with earlier studies suggesting a role of synaptobrevin-2 in endocytosis, these results indicate that the machinery for fast synchronous release couples fusion to retrieval and regulates the kinetics of endocytosis in a Ca 2+ -dependent manner.