English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Open

Presents the pdf analysis as premium feature

PDF Analysis

Insights

Presents the summarisation as premium feature

Summarisation

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

The lipid droplet (LD) fraction of milk has attracted special attention because it supplies preformed lipids for neonatal development, and the assembled LDs are secreted by a unique apocrine mechanism. Because many aspects of this key process remain uncharacterized, we developed a facile method for the intravital imaging of mammary cells in transgenic mice that express fluorescently tagged marker proteins. Using these techniques, we describe the first kinetic analysis of LD growth and secretion at peak lactation in real time. LD transit from basal to apical regions was slow (0–2 μm/min) and frequently intermittent. Droplets grew by the fusion of preexisting droplets, with no restriction on the size of fusogenic partners. Most droplet expansion took several hours and occurred in apical nucleation centers, either close to or in association with the apical surface. Droplets even continued to expand as they were emerging from the cell. Contrary to expectations, LDs attached to the apical plasma membrane but still associated with the cytoplasm were released after oxytocin-mediated contraction of the myoepithelium. Thus milk LD secretion is an intermittently regulated process. This novel procedure will have broad application for investigating trafficking events within the mammary epithelium in real time.

molecular biology of the cell

Kinetics of milk lipid droplet transport, growth, and secretion revealed by intravital imaging: lipid droplet release is intermittently stimulated by oxytocin