Open AccessMicroRNA-185 oscillation controls circadian amplitude of mouse Cryptochrome 1 via translational regulation
Author(s) -
Kyung Ha Lee,
Sung Hoon Kim,
Hwa Rim Lee,
Wanil Kim,
Do Yeon Kim,
Jae Cheon Shin,
Seung Hee Yoo,
KyongTai Kim
Publication year - 2013
Publication title -
molecular biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.463
H-Index - 225
eISSN - 1939-4586
pISSN - 1059-1524
DOI - 10.1091/mbc.e12-12-0849
Subject(s) - drosha , biology , cryptochrome , gene knockdown , three prime untranslated region , microrna , circadian rhythm , microbiology and biotechnology , suprachiasmatic nucleus , dicer , circadian clock , messenger rna , post transcriptional regulation , translation (biology) , untranslated region , translational regulation , clock , regulation of gene expression , gene expression , rna interference , genetics , gene , rna , endocrinology
Mammalian circadian rhythm is observed not only at the suprachiasmatic nucleus, a master pacemaker, but also throughout the peripheral tissues. Investigation of the regulation of clock gene expression has mainly focused on transcriptional and posttranslational modifications, and little is known about the posttranscriptional regulation of these genes. In the present study, we investigate the role of microRNAs (miRNAs) in the posttranscriptional regulation of the 3′-untranslated region (UTR) of the mouse Cryptochrome 1 (mCry1) gene. Knockdown of Drosha, Dicer, or Argonaute2 increased mCry1-3′UTR reporter activity. The presence of the miRNA recognition element of mCry1 that is important for miR-185 binding decreased mCRY1 protein, but not mRNA, level. Cytoplasmic miR-185 levels were nearly antiphase to mCRY1 protein levels. Furthermore, miR-185 knockdown elevated the amplitude of mCRY1 protein oscillation. Our results suggest that miR-185 plays a role in the fine-tuned regulation of mCRY1 protein expression by controlling the rhythmicity of mCry1 mRNA translation.