Open AccessOntogenetic Changes in Blood Osmolality During the Postembryonic Development of Zebrafish (Danio rerio)
Author(s) -
Guy Charmantier,
Maï Nguyen-Chi,
Georges Lutfalla
Publication year - 2022
Publication title -
zebrafish
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.722
H-Index - 46
eISSN - 1557-8542
pISSN - 1545-8547
DOI - 10.1089/zeb.2021.0075
Subject(s) - danio , zebrafish , biology , metamorphosis , ontogeny , osmoregulation , larva , osmotic concentration , zoology , anatomy , ecology , endocrinology , biochemistry , salinity , gene
The zebrafish Danio rerio is a teleost model species widely used in developmental genetics, biomedical studies, toxicology, and drug screening. Despite the interest of this species in research, little is known through indirect observations about its blood osmolality, which is a key parameter for diverse experiments. In this study, we directly measured blood osmolality using nano-osmometry at different stages of zebrafish postembryonic development. We found that blood osmolality is close to 240 mOsm·kg -1 in early larvae. It progressively increased to ∼270 mOsm·kg -1 during the larval development before reaching ∼300 mOsm·kg -1 after metamorphosis in juveniles and later in adults. These ontogenetic changes in blood osmolality illustrate the physiological changes in osmoregulation associated with postembryonic development, including metamorphosis. These values are of practical interest for adjusting the osmolality of fixatives and cell and tissue culture media for research using zebrafish as a model.