Open AccessListeria monocytogenesDelivery of HPV-16 Major Capsid Protein L1 Induces Systemic and Mucosal Cell-Mediated CD4+and CD8+T-Cell Responses After Oral Immunization
Author(s) -
Waleed Mustafa,
Paulo Maciag,
Zhen-Kun Pan,
Jessica L. Weaver,
Yuhong Xiao,
Stuart N. Isaacs,
Yvonne Paterson
Publication year - 2009
Publication title -
viral immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.777
H-Index - 61
eISSN - 1557-8976
pISSN - 0882-8245
DOI - 10.1089/vim.2008.0071
Subject(s) - capsid , virology , listeria monocytogenes , immunization , vaccinia , listeriolysin o , biology , cd8 , morbillivirus , antibody , immunology , vaccination , virus , microbiology and biotechnology , recombinant dna , measles virus , immune system , measles , listeria , biochemistry , genetics , bacteria , gene
Neutralizing antibodies are thought to be required at mucosal surfaces to prevent human papillomavirus (HPV) transmission. However, the potential for cell-mediated immunity in mediating protection against HPV infection has not been well explored. We generated recombinant Listeria monocytogenes (Lm) constructs that secrete listeriolysin O (LLO) fused with overlapping N-terminal (LLO-L1(1-258)) or C-terminal (LLO-L1(238-474)) fragments of HPV type 16 major capsid protein L1 (HPV-16-L1). Oral immunization of mice with either construct induced IFN-gamma-producing CD8+ and CD4+ T cells in the spleen and in the Peyer's patches with the C-terminal construct. Oral immunization with both constructs resulted in diminished viral titers in the cervix and uterus of mice after intravaginal challenge with vaccinia virus expressing HPV-16-L1.