Open AccessLED-Based Photoacoustic Imaging for Monitoring Angiogenesis in Fibrin Scaffolds
Author(s) -
Yunhao Zhu,
Xiaofang Lu,
Xiaoxiao Dong,
Jie Yuan,
Mario L. Fabiilli,
Xueding Wang
Publication year - 2019
Publication title -
tissue engineering. part c methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.846
H-Index - 70
eISSN - 1937-3392
pISSN - 1937-3384
DOI - 10.1089/ten.tec.2019.0151
Subject(s) - biomedical engineering , neovascularization , photoacoustic imaging in biomedicine , speckle pattern , angiogenesis , regenerative medicine , preclinical imaging , chemistry , medicine , computer science , in vivo , optics , computer vision , biology , biochemistry , physics , cell , microbiology and biotechnology
Noninvasive imaging techniques provide insight into physiology that is complementary to tissue morphology obtained by invasive histology. Optical imaging techniques, such as laser speckle contrast analysis, are used in vivo to longitudinally evaluate vascularization. Despite their high spatial resolution, these techniques have a limited imaging depth. In this study, we demonstrate how a dual LED-based photoacoustic (PA) and ultrasound system can delineate changes in perfusion at depth within scaffolds containing basic fibroblast growth factor. Perfusion changes detected by PA corroborated with vessel density. PA imaging could be a noninvasive and sensitive method for evaluating vascularization at depth in larger constructs.