Open AccessBioreactor System to Perfuse Mesentery Microvascular Networks and Study Flow Effects During Angiogenesis
Author(s) -
Jessica M. Motherwell,
Maximillian Rozenblum,
Prasad V. G. Katakam,
Walter L. Murfee
Publication year - 2019
Publication title -
tissue engineering. part c methods
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.846
H-Index - 70
eISSN - 1937-3392
pISSN - 1937-3384
DOI - 10.1089/ten.tec.2019.0119
Subject(s) - angiogenesis , ex vivo , neovascularization , tissue remodeling , in vivo , microbiology and biotechnology , biology , cancer research , immunology , inflammation
Microvascular remodeling, or angiogenesis, plays a central role in multiple pathological conditions, including cancer, diabetes, and ischemia. Tissue-engineered in vitro models have emerged as tools to elucidate the mechanisms that drive the angiogenic process. However, a major challenge with model development is recapitulating the physiological complexity of real microvascular networks, including incorporation of the entire vascular tree and hemodynamics. This study establishes a bioreactor system that incorporates real microvascular networks with physiological flow as a novel ex vivo tissue culture model, thereby providing a platform to evaluate angiogenesis in a physiologically relevant environment.