Open AccessLytic Bacteriophages Against Bacterial Biofilms Formed by Multidrug-Resistant Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus Isolated from Burn Wounds
Author(s) -
Roja Rani Pallavali,
Vijaya Lakshmi Degati,
Venkata Ramireddy Narala,
Kiran Kumar Velpula,
Suresh Yenugu,
Vijaya Raghava Prasad Durbaka
Publication year - 2021
Publication title -
phage
Language(s) - English
Resource type - Journals
eISSN - 2641-6549
pISSN - 2641-6530
DOI - 10.1089/phage.2021.0004
Subject(s) - microbiology and biotechnology , biofilm , lytic cycle , klebsiella pneumoniae , pseudomonas aeruginosa , staphylococcus aureus , escherichia coli , bacteriophage , bacteria , biology , multiple drug resistance , antibiotics , klebsiella , colony forming unit , virology , virus , biochemistry , genetics , gene
Background: Use of bacteriophages as antibiofilm agents to tackle multidrug-resistant bacteria has gained importance in recent years. Materials and Methods: In this study, biofilm formation by Staphylococcus aureus , Pseudomona aeruginosa , Klebsiella pneumoniae , and Escherichia coli under different growth conditions was studied. Furthermore, the ability of bacteriophages to inhibit biofilm formation was analyzed. Results: Under dynamic growth condition, wherein the medium is renewed for every 12 h, the amount of biomass produced and log 10 colony-forming unit counts of all bacterial species studied was highest when compared with other growth conditions tested. Biomass of biofilms produced was drastically reduced when incubated for 2 or 4 h with bacteriophages vB_SAnS_SADP1, vB_PAnP_PADP4, vB_KPnM_KPDP1, and vB_ECnM_ECDP3. Scanning electron microscopy and confocal laser scanning microscopy analyses indicated that the reduction in biomass was due to the lytic action of the bacteriophages. Conclusions: Results of our study reinforce the concept of developing bacteriophages as alternatives to antibiotics to treat bacterial infections.