Open AccessGenome Sequence and Characterization ofAcinetobacterPhage DMU1
Author(s) -
Bailey M. Pehde,
Devon Niewohner,
F. E. Keomanivong,
Michael D. Carruthers
Publication year - 2021
Publication title -
phage
Language(s) - English
Resource type - Journals
eISSN - 2641-6549
pISSN - 2641-6530
DOI - 10.1089/phage.2020.0043
Subject(s) - siphoviridae , acinetobacter baumannii , biology , phage therapy , bacteriophage , genome , acinetobacter , microbiology and biotechnology , comparative genomics , genetics , genomics , gene , bacteria , pseudomonas aeruginosa , antibiotics , escherichia coli
Background: There has been a recent resurgence of research on the characterization of Acinetobacter phage for therapeutic use due to the morbidity and mortality associated with treatment failures in cases of multidrug-resistant Acinetobacter baumannii infections. Materials and Methods: A bacteriophage isolated from activated sludge that targets A. baumannii ATCC19606 was characterized by electron microscopy, genome sequencing, comparative genomics, and a host range analysis. Results: The morphology of Acinetobacter phage DMU1 resembles phages in Siphoviridae . Comparative genomic and phylogenetic analyses reveal that DMU1 is a siphophage and is most closely related to Acinetobacter phage SH-Ab 15497. Out of the strains tested, DMU1 was found to only infect A. baumannii strains ATCC19606 and ATCC17978. Conclusion: Acinetobacter phage DMU1 belongs to the Siphoviridae family and is most closely related to Acinetobacter phage SH-Ab 15497. Small-scale host-range analysis of DMU1 indicates a host range that is likely limited to specific A. baumannii strains.