A Dynamic Method for Broad-Spectrum Bacteriophage Cocktail Formulation Against Poultry-AssociatedSalmonella enterica | Zendy

Thomas Brenner | Zendy; Karen Fong | Zendy; Spencer Lee | Zendy; Siyun Wang | Zendy

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A Dynamic Method for Broad-Spectrum Bacteriophage Cocktail Formulation Against Poultry-AssociatedSalmonella enterica

Author(s) -

Thomas Brenner,

Karen Fong,

Spencer Lee,

Siyun Wang

Publication year - 2020

Publication title -

phage

Language(s) - English

Resource type - Journals

eISSN - 2641-6549

pISSN - 2641-6530

DOI - 10.1089/phage.2020.0002

Subject(s) - salmonella enterica , lytic cycle , salmonella , bacteriophage , serotype , biology , microbiology and biotechnology , antimicrobial , phage therapy , myoviridae , broad spectrum , highly pathogenic , poultry farming , agar , virology , bacteria , virus , escherichia coli , chemistry , genetics , gene , ecology , influenza a virus subtype h5n1 , combinatorial chemistry

Background: Poultry products are the largest food category linked to salmonellosis in Canada. Bacteriophages (phages) have been proposed as a novel antimicrobial in the poultry industry due to their documented ubiquity, efficacy, and safety benefits. Materials and Methods: A library of 78 lytic phages was rapidly screened against 50 prominent poultry-associated Salmonella enterica isolates procured from British Columbia, Canada. Results: A phage cocktail was successfully formulated using only three sewage-isolated phages (SE4, SE13, and SE20) to achieve broad-spectrum antimicrobial efficacy across all S. enterica serovars. Highly promising phages were also characterized using one-step growth curves and transmission electron microscopy. Conclusion: Relative host efficiency is a new agar-based semiquantitative metric developed here for the rapid comparison of different phages against a panel of known bacterial targets.

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