Open AccessGenome Sequence and Characterization of Coliphage vB_Eco_SLUR29
Author(s) -
Ibrahim Besler,
Pavelas Sazinas,
Charlotte Harrison,
Lucy Gan,
Tamsin Redgwell,
Slawomir Michniewski,
Steven P.T. Hooton,
Jon L. Hobman,
Andrew Millard
Publication year - 2020
Publication title -
phage
Language(s) - English
Resource type - Journals
eISSN - 2641-6549
pISSN - 2641-6530
DOI - 10.1089/phage.2019.0009
Subject(s) - coliphage , siphoviridae , biology , subfamily , genome , lytic cycle , multiplicity of infection , genetics , phylogenetic tree , bacteriophage , virology , escherichia coli , gene , virus
Background: Bacteriophages that infect Escherichia coli are relatively easily isolated, with >600 coliphage genomes sequenced to date. Despite this there is still much to be discovered about the diversity of coliphage genomes. Materials and Methods: Within this study, we isolated a coliphage from cattle slurry collected from a farm in rural England. Results: Transmission electron microscopy identified the phage as member of the Siphoviridae family. Phylogenetic analysis and comparative genomics further placed it within the subfamily Tunavirinae and forms part of a new genus. Conclusions: Characterization of the lytic properties of vB_Eco_SLUR29 reveals that it is rapidly able to lyse its host when infected at high multiplicity of infection, but not at low multiplicity of infection.