Open AccessComparison of GFAP and UCH-L1 Measurements from Two Prototype Assays: The Abbott i-STAT and ARCHITECT Assays
Author(s) -
Frederick K. Korley,
Saul A. Datwyler,
Sonia Jain,
Xiaoying Sun,
Gangamani S. Beligere,
Raj Chandran,
Jaime A. Marino,
Beth McQuiston,
Hongwei Zhang,
Krista L. Caudle,
Kevin Wang,
Ava M. Puccio,
David O. Okonkwo,
John K. Yue,
Sabrina Taylor,
Amy J. Markowitz,
Geoffrey T. Manley,
Ramon DiazArrastia
Publication year - 2021
Publication title -
neurotrauma reports
Language(s) - English
Resource type - Journals
ISSN - 2689-288X
DOI - 10.1089/neur.2020.0037
Subject(s) - interquartile range , glial fibrillary acidic protein , biomarker , medicine , chemistry , surgery , pathology , biochemistry , immunohistochemistry
Glial fibrillary acidic protein (GFAP) and ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) may aid in the evaluation of traumatic brain injury (TBI). The objective of this analysis was to compare GFAP and UCH-L1 values measured using a handheld device compared with a core laboratory platform. We analyzed plasma samples from patients with TBI and healthy controls enrolled in the T ransforming R esearch a nd C linical K nowledge in TBI (TRACK-TBI) cohort study. GFAP and UCH-L1 were measured twice in each subject using prototype assays, first with the Abbott i-STAT™ handheld device, and second with the Abbott ARCHITECT ® platform. We then quantified the agreement in biomarker values obtained using these two methods. GFAP and UCH-L1 were measured twice in 570 and 572 samples, respectively. GFAP values measured by the ARCHITECT platform (median 143.3 [interquartile range (IQR): 19.8–925.8] pg/mL) were higher than values measured by the i-STAT (median 116.0 [IQR: 9.2–856.5] pg/mL). GFAP values from the two platforms were strongly correlated ( p = 0.985). Similarly, UCH-L1 values measured by the ARCHITECT platform (median 163.9 [IQR: 82.5–412.4] pg/mL) were higher than values measured by the i-STAT (median 122.5 [IQR: 63.0–297.3] pg/mL). UCH-L1 values from the two platforms were strongly correlated ( p = 0.933). Passing-Bablok regression equations were developed to estimate the relationship between the two platforms, specifically to predict i-STAT values from the ARCHITECT platform. GFAP and UCH-L1 values measured using the prototype assays on the Abbott i-STAT and ARCHITECT platforms are strongly correlated and values from either platform may be converted to the other.