Open AccessOccurrence and Antimicrobial Susceptibility Profile ofSalmonellaspp. in Raw and Ready-To-Eat Foods andCampylobacterspp. in Retail Raw Chicken Meat in Transylvania, Romania
Author(s) -
E. Tîrziu,
Gabriel Bărbălan,
Adriana Morar,
V. Herman,
Romeo Teodor Cristina,
Kálmán Imre
Publication year - 2020
Publication title -
foodborne pathogens and disease
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.833
H-Index - 69
eISSN - 1556-7125
pISSN - 1535-3141
DOI - 10.1089/fpd.2019.2738
Subject(s) - salmonella , nalidixic acid , campylobacter , tetracycline , ciprofloxacin , microbiology and biotechnology , biology , ampicillin , antimicrobial , campylobacter coli , food science , antibiotics , bacteria , genetics
The survey was undertaken to investigate the presence and antimicrobial susceptibility profile of Salmonella spp. in raw and ready-to-eat (RTE) foods, and Campylobacter spp. in the retail raw chicken meat collected in two counties of Transylvania, Romania. A total of 13.1% (51/388) of the examined food samples were found to be Salmonella positive, with a distribution of 14.7% (48/326) in the raw food (i.e., pork, chicken carcass, and shell egg) and 4.8% (3/62) in the RTE samples (i.e., sausages, but not ham and salami), respectively. These differences were statistically significant ( p = 0.034). The isolates were serotyped as Salmonella Infantis ( n = 19), Salmonella Typhimurium ( n = 11) Salmonella Rissen ( n = 8), Salmonella Derby ( n = 3), Salmonella Enteritidis ( n = 3), Salmonella Bredeney ( n = 2), Salmonella Brandenburg ( n = 1), Salmonella Gloucester ( n = 1), Salmonella Goldcoast ( n = 1), Salmonella Kottbus ( n = 1), and Salmonella Ruzizi ( n = 1). Campylobacter strains were present in 29.4% (10/34) of the investigated chicken samples, and the identified species were Campylobacter coli (70%) and C. jejuni (30%). From the 14 tested antimicrobials, the Salmonella isolates were resistant against azithromycin (88.2%), tetracycline (54.9%), sulfamethoxazole (54.9%), ciprofloxacin (45.1%), nalidixic acid (43.1%), ampicillin (35.3%), chloramphenicol (33.3%), tigecycline (25.5%), cefotaxime (13.7%), colistin (13.7%), trimethoprim (7.8%), and gentamicin (2%), resulting in the expression of 21 multidrug-resistant (MDR) profiles. Of 10 Campylobacter isolates, 80% were resistant to ciprofloxacin and nalidixic acid, 40% to tetracycline, and 10% to streptomycin and erythromycin, respectively. Our findings indicate that Romanian isolates of Salmonella spp. and Campylobacter spp., contaminating animal-origin foods, can exhibit MDR patterns, representing a public health risk.