Open AccessDiversification of the CRISPR Toolbox: Applications of CRISPR-Cas Systems Beyond Genome Editing
Author(s) -
Sarah Balderston,
Gabrielle Clouse,
Juan-José Ripoll,
Grace K Pratt,
Giedrius Gasiūnas,
Jens-Ole Bock,
Eric P. Bennett,
Kiana Aran
Publication year - 2021
Publication title -
the crispr journal
Language(s) - English
Resource type - Journals
eISSN - 2573-1602
pISSN - 2573-1599
DOI - 10.1089/crispr.2020.0137
Subject(s) - crispr , genome editing , toolbox , computational biology , biology , genome , computer science , gene , genetics , programming language
The discovery of CRISPR has revolutionized the field of genome engineering, but the potential of this technology is far from reaching its limits. In this review, we explore the broad range of applications of CRISPR technology to highlight the rapid expansion of the field beyond gene editing alone. It has been demonstrated that CRISPR technology can control gene expression, spatiotemporally image the genome in vivo , and detect specific nucleic acid sequences for diagnostics. In addition, new technologies are under development to improve CRISPR quality controls for gene editing, thereby improving the reliability of these technologies for therapeutics and beyond. These are just some of the many CRISPR tools that have been developed in recent years, and the toolbox continues to diversify.