Open AccessWhich Antioxidant System Shapes Intracellular H2O2 Gradients?
Author(s) -
Natalia M. Mishina,
Yu. A. Bogdanova,
Yulia G. Ermakova,
Anastasiya S. Panova,
Daria A. Kotova,
Dmitry S. Bilan,
Benjamin Steinhorn,
Elias S.J. Arnér,
Thomas Michel,
Vsevolod V. Belousov
Publication year - 2019
Publication title -
antioxidants and redox signaling
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.277
H-Index - 190
eISSN - 1557-7716
pISSN - 1523-0864
DOI - 10.1089/ars.2018.7697
Subject(s) - intracellular , antioxidant , glutathione , hydrogen peroxide , thioredoxin , hela , chemistry , microbiology and biotechnology , biophysics , biochemistry , biology , oxidative stress , enzyme , cell
Cellular antioxidant systems control the levels of hydrogen peroxide (H 2 O 2 ) within cells. Multiple theoretical models exist that predict the diffusion properties of H 2 O 2 depending on the rate of H 2 O 2 generation and amount and reaction rates of antioxidant machinery components. Despite these theoretical predictions, it has remained unknown how antioxidant systems shape intracellular H 2 O 2 gradients. The relative role of thioredoxin (Trx) and glutathione systems in H 2 O 2 pattern formation and maintenance is another disputed question. Here, we visualized cellular antioxidant activity and H 2 O 2 gradients formation by exploiting chemogenetic approaches to generate compartmentalized intracellular H 2 O 2 and using the H 2 O 2 biosensor HyPer to analyze the resulting H 2 O 2 distribution in specific subcellular compartments. Using human HeLa cells as a model system, we propose that the Trx system, but not the glutathione system, regulates intracellular H 2 O 2 gradients. Antioxid. Redox Signal. 31, 664-670.