Open AccessFabrication of a novel fluorescent polyacrylonitrile electrospun nanofiber for DNA-based optical biosensing of microRNA-21
Author(s) -
Homeira Zare Chavoshy,
Razieh Ghasemi
Publication year - 2020
Publication title -
nano express
Language(s) - English
Resource type - Journals
ISSN - 2632-959X
DOI - 10.1088/2632-959x/ab96b5
Subject(s) - nanofiber , polyacrylonitrile , electrospinning , fluorescence , covalent bond , materials science , polymer , biosensor , nitrile , polymer chemistry , nanotechnology , chemistry , organic chemistry , physics , quantum mechanics , composite material
In this study, an optical platform based on fluorescent nanofiber was developed for detection of microRNA-21 as a biomarker of cancerous cells. The fluorescent polyacrylonitrile nanofiber was fabricated using electrospinning method. The surfaces of nanofiber were treated by NaOH to convert nitrile groups to carboxyl moieties. Activation of these carboxyl groups by EDC-sulfo NHS coupling agents was done and then the covalent bonding between COOH groups of nanofiber and –NH 2 groups of amino-probe was formed. The results of SEM, XRD, FTIR, and fluorescence microscopy confirmed that the process of fluorescent nanofiber synthesis was successfully performed. Hybridization of probe and microRNA-21 showed that the capture efficiency of this fluorescent nanofiber was 1 pmol μ l −1 and these nanofibers have the potential to be used as optical sensors for detection of microRNA-21.