Open AccessCharacterization of normal and deformed red blood cells using simulated differential photoacoustic cross-section spectral data
Author(s) -
Anuj Kaushik,
Ratan K. Saha
Publication year - 2021
Publication title -
journal of physics communications
Language(s) - English
Resource type - Journals
ISSN - 2399-6528
DOI - 10.1088/2399-6528/abebd0
Subject(s) - ellipsoid , cylinder , shape factor , mathematics , mathematical analysis , geometry , approximation error , inverse , physics , astronomy
Frequency dependent differential photoacoustic cross-section (DPACS) over a large frequency band (100–1000 MHz) has been computed and subsequently, morphological parameters of photoacoustic (PA) source have been quantified. Green’s function method has been employed for computing the DPACS for a series of ellipsoidal droplets (with varying aspect ratio), Chebyshev particles (with different waviness (n) and deformation ( ϵ ) parameters), healthy red blood cell (RBC) and cells suffering from hereditary disorders (spherocytosis, elliptocytosis and stomatocytosis). The tri-axial ellipsoid form factor (TAEFF), finite cylinder form factor (CFF) and toroid form factor (TFF) models have been used to fit the DPACS spectrum to obtain size and shape information of the PA source. The TAEFF model estimates the shape parameters of the ellipsoidal droplets accurately (error 15%) for other deformed RBCs. The inverse problem framework may motivate to develop a PA-based technology to assess single cell morphology.