Open AccessReal-time fluorescence sensing of single photoactive proteins using silver nanowires
Author(s) -
Karolina Sulowska,
Kamil Wiwatowski,
Maciej Ćwierzona,
JoaniedziółkaJönsson,
Sebastian Maćkowski
Publication year - 2020
Publication title -
methods and applications in fluorescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 25
ISSN - 2050-6120
DOI - 10.1088/2050-6120/aba7cb
Subject(s) - fluorescence , nanowire , plasmon , materials science , fluorescence microscope , surface plasmon resonance , nanotechnology , photoactive layer , chemistry , optoelectronics , optics , nanoparticle , physics , energy conversion efficiency , polymer solar cell
We demonstrate that single functionalized silver nanowires form a geometric platform suitable for efficient real-time detection of single photoactive proteins. By collecting series of images using wide-field fluorescence microscopy, events of single protein attachment can be distinguished with the signal to noise ratio further improved by fluorescence enhancement due to plasmon excitations in the nanowires. The enhancement is evidenced by strong shortening of the fluorescence decay of single photoactive proteins conjugated to the silver nanowires.