Open AccessToward a neurospheroid niche model: optimizing embedded 3D bioprinting for fabrication of neurospheroid brain-like co-culture constructs
Author(s) -
YiChen Ethan Li,
Yasamin A. Jodat,
Roya Samanipour,
Giulio Zorzi,
Kai Zhu,
Minoru Hirano,
Karen L. Chang,
Adnan Arnaout,
Shabir Hassan,
Navneet Matharu,
Ali Khademhosseini,
Mina Hoorfar,
Su Ryon Shin
Publication year - 2020
Publication title -
biofabrication
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.328
H-Index - 80
eISSN - 1758-5090
pISSN - 1758-5082
DOI - 10.1088/1758-5090/abc1be
Subject(s) - 3d bioprinting , spheroid , neural stem cell , neural cell , 3d cell culture , materials science , computer science , nanotechnology , tissue engineering , biomedical engineering , neuroscience , cell culture , cell , stem cell , biology , microbiology and biotechnology , engineering , genetics
A crucial step in creating reliable in vitro platforms for neural development and disorder studies is the reproduction of the multicellular three-dimensional (3D) brain microenvironment and the capturing of cell-cell interactions within the model. The power of self-organization of diverse cell types into brain spheroids could be harnessed to study mechanisms underlying brain development trajectory and diseases. A challenge of current 3D organoid and spheroid models grown in petri-dishes is the lack of control over cellular localization and diversity. To overcome this limitation, neural spheroids can be patterned into customizable 3D structures using microfabrication. We developed a 3D brain-like co-culture construct using embedded 3D bioprinting as a flexible solution for composing heterogenous neural populations with neurospheroids and glia. Specifically, neurospheroid-laden free-standing 3D structures were fabricated in an engineered astrocyte-laden support bath resembling a neural stem cell niche environment. A photo-crosslinkable bioink and a thermal-healing supporting bath were engineered to mimic the mechanical modulus of soft tissue while supporting the formation of self-organizing neurospheroids within elaborate 3D networks. Moreover, bioprinted neurospheroid-laden structures exhibited the capability to differentiate into neuronal cells. These brain-like co-cultures could provide a reproducible platform for modeling neurological diseases, neural regeneration, and drug development and repurposing.