Open AccessDetermination of structure and anticancer activity of MM2 compound, isolated from endophytic fungus Aspergillus terreus-RTN3 of Alpinia chinensis Rosc.
Author(s) -
ThanhSang Vo,
D. N. Nguyen,
Van Thuy Nguyen
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/991/1/012045
Subject(s) - hela , aspergillus terreus , dpph , chemistry , zingiberaceae , plant use of endophytic fungi in defense , bioactive compound , antioxidant , cancer cell , hep g2 , staphylococcus aureus , singlet oxygen , stereochemistry , rhizome , cancer , biochemistry , traditional medicine , biology , organic chemistry , cell , medicine , in vitro , oxygen , botany , bacteria , genetics
The study has isolated endogenous A. terreus -RTN3 strain from young stems of Alpinia chinensis Rosc.-Zingiberaceae family. Cultivating and extracting compounds containing metabolites that are resistant to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA). Determining the number of antibacterial compounds in the crude extract by thin-layer chromatography and vacuum liquid chromatography (VLC), the research has isolated pure MM 2 compound with high antioxidant activity and spectral resolution to find the structure compound MM 2 (Methyl 2-((4-amino-2-bromo-3-methyl-5-thioxocyclopenta-1,3-dien-1-yl) oxygen) -4-hydroxy-6-methoxybenzoate). This is a new compound that has not been announced in any works in the world. MM2 compound has a high antioxidant activity, the ability to catch α, α-diphenyl-β-picrylhydrazyl (DPPH) free radicals increases linearly by concentration, at concentration 400ppm of MM 2 is capable of catching over 75% of DPPH free radicals. The MM 2 compound also has the ability to inhibit 4 test cell lines: breast cancer (MCF-7), cervical cancer (Hela), liver cancer (Hep G2) and lung cancer (NCI-H460). The results showed that MM 2 compound’s ability to inhibit cancer cell lines increased linearly, at concentrations of 100 µg/ml of MM 2 compound, which inhibited nearly 80% of cancer cell lines and was high most in liver cancer cell line HepG2 (at a concentration of 100 µg/ml inhibits 86.82% of liver cancer cells HepG2).