Open AccessThe addition effect of Pseudomonas aeruginosa on biodegradation of methyl orange dye by brown-rot fungus Fomitopsis pinicola
Author(s) -
Adi Setyo Purnomo,
Driyanti Rahayu,
Refdinal Nawfa,
Surya Rosa Putra
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/959/1/012008
Subject(s) - chemistry , benzene , pseudomonas aeruginosa , incubation , hydroxylation , demethylation , microbiology and biotechnology , medicinal chemistry , organic chemistry , bacteria , biochemistry , enzyme , biology , genetics , gene expression , gene , dna methylation
The addition effect of Pseudomonas aeruginosa on methyl orange (MO) biodecolorization by Fomitopsis pinicola had been investigated. P. aeruginosa was added into F. pinicola culture at 2, 4, 6, 8, 10 mL (1 mL × 5.05 χ 10 12 CFU). The addition of 4 ml of P. aeruginosa showed the highest MO biodecolorization approximately 99.53%, while by F. pinicola only was 77.22% in potato dextrose broth (PDB) medium for 7 days incubation. Four metabolites were detected from MO degradation by mixed cultures such as 4-(4-(dimethylimino) hydroxy-cyclohexa-2,5-dien-1-ylidene) hydrazinyl) phenolate; 4-(hydroxy-4-iminio-cyclohexa-2,5-dien-1-ylidene) hydrazinyl) benzene sulfonate; 4-(4-(dimethylimino) methoxy-cyclohexa-2,5-dien-1-ylidene) hydrazinyl) hydroxy-benzene sulfonate; and 4-(hydroxy-4-iminio-cyclohexa-2,5-dien-1-ylidene) hydrazinil) hidroxy-benzene sulfonate. The mixed cultures transformed MO via three pathways: (1) desulfonylation, (2) demethylation, and (3) hydroxylation. These results indicated that P. aeruginosa can enhance MO biodecolorization by F. pinicola.