Open AccessEnhanced cellular uptake and anti-cancer potentials of gold nanoparticles conjugated with cell penetration peptide against lung cancer cells
Author(s) -
Ali G. Al-Dulimi,
Ali Z. AlSaffar,
Ghassan M. Sulaiman
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/928/6/062025
Subject(s) - cytotoxicity , cell culture , peptide , colloidal gold , mtt assay , cancer cell , chemistry , conjugated system , cell , cancer research , cell growth , cell penetrating peptide , cancer , in vitro , penetration (warfare) , biophysics , nanoparticle , nanotechnology , materials science , biochemistry , medicine , biology , genetics , organic chemistry , operations research , engineering , polymer
Gold nanoparticles (GNPs) are often conjugated in the biomedical field with biocompatible peptides, although the effect of biocompatible GNP peptides on cellular responses is still not clearly understood. In the current study, GNPs with / without peptide were used as model probes to investigate the cytotoxicity to the human lung cancer cell line (A457) and human normal breast epithelial cell line (HBL-100). GNPs and GNPs-RGD preparation was confirmed and characterized using UV – VIS spectrophotometer, FE-SEM, FTIR, and TEM. The anticancer effect to A457 cell line was estimated using MTT assay. Our results show that the GNP-RGD had found significant tumor targeted efficacy and decrease in proliferation of A457 cell line compared with HBL-100 which appeared normal growth. Overall, our finding suggests a potential therapeutic effect of GNPs-RGD as a novel anti-cancer drug to be further developed and offer a beneficial targeting therapy.