Open AccessHeterologous Expression and Enzymatic Properties of Exocellulase CelA
Author(s) -
Ming Su,
Jiachao Rong,
Yang Zhang,
Peng Qu,
Yusong Zhu
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/914/1/012012
Subject(s) - genbank , bacillus licheniformis , heterologous expression , gene , heterologous , accession number (library science) , recombinant dna , strain (injury) , biology , genetics , microbiology and biotechnology , bacteria , anatomy , bacillus subtilis
The gene of glycosyl hydrolase CeIA is derived from Bacillus licheniformis and the GenBank accession number is AAU40776.1. The sequence analysis of this gene was carried out by online software, and the celA gene was cloned from the genome of the strain by homologous amplification. The gene was ligated into the pMAL-c2x vector, transformed into E. coli Transetta for heterologous expression, and the catalytic properties of the recombinant enzyme CeIA were studied.