Open AccessIsolation and Identification of Local Bactria Produced from Soil-Borne Urease
Author(s) -
Nawar Ali,
Mahdi O. Karkush,
H. H. Al Haideri
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/901/1/012035
Subject(s) - urease , 16s ribosomal rna , nutrient agar , urea , bacteria , agar , isolation (microbiology) , biology , microbiology and biotechnology , agar plate , strain (injury) , ribosomal rna , chemistry , hydrolysis , food science , biochemistry , gene , genetics , anatomy
The hydrolysis of urea by the enzyme urease is significant for increasing the irroles in human pathogenicity, biocementation, soil fertilizer, and subsequently in soil improvement. This study devoted to the isolation of urease from urea-rich soil samples collected from seven different locations. Isolation of the various bacterial species was conducted using nutrient agar. The identity of isolated urease was based on morphological characteristics and standard microbiological and biochemical procedures. The urease producing strains of bacteria were obtained using the urease hydrolysis test. The bacterial isolates produced from soil samples collected from different environments and treated by different morphological processes helped in precipitation of large calcium carbonate (CaCO 3 ) crystal aggregates precipitated within bacterial colonies grown on agar. The different microbial species and functional attributes produced striking differences in the morphology of precipitated crystals. The phylogenetic sequencing of 16S ribosomal RNA genes produced several isolates that are mostly related to the Bacillus group. One strain of promising results was selected and the environmental and nutritional conditions were characterized. The growth curve of the selected strain with an optimized condition was investigated.