Open AccessImpact of Gold nanoparticles sizes and concentrations on the Rhabdomyosarcoma cells
Author(s) -
Sura M. Sawalha,
Eman Ghadhban,
Ziad T. Al-Dahan
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/870/1/012022
Subject(s) - chemistry , bromide , colloidal gold , cytotoxic t cell , absorbance , rhabdomyosarcoma , nuclear chemistry , membrane , biophysics , cytotoxicity , particle size , nanoparticle , sulforhodamine b , cell culture , chromatography , in vitro , nanotechnology , biochemistry , materials science , inorganic chemistry , biology , medicine , sarcoma , pathology , genetics
The cytotoxic effect of gold nanoparticles (GNPs) on Rhabdomyosarcoma cells (RMS or RD cell line) has been studied by using Methyl Thiazolyl tetrazolium (MTT) assay. Two sizes of the synthesized GNPs (61nm and 71 nm) functionalized with cetyltrimethylammonium bromide with different concentrations of 3.6 ml of 5 ml CTAB solution were used in the current research. The RD cell line was exposed to these particles for 48 hours. The findings showed that the cytotoxic effects of GNPs appeared to depend on concentration. for both sizes at higher concentration, the inhibition rate of IR absorbance was significantly decreased due to GNPs agglomeration on the cell membrane. Lower concentrations of GNPs internalize the cytoplasmic membrane individually by diffusion. Moreover, average particle diameters of 61nm showed a significant inhibition rate of more than 71nm at a lower concentration.