Open AccessSynthesis of ZnO-Ag with Clove Oil Using Ultrasonication Method and Its Antibiofilm Activity Against Klebsiella pneumoniae
Author(s) -
Khoirun Nisyak,
Yulianto Ade Prasetya,
A’yunil Hisbiyah,
Elvina Dhiaul Iftitah,
Arie Srihardyastutie
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/833/1/012081
Subject(s) - orthorhombic crystal system , sonication , diffractometer , crystallinity , scanning electron microscope , nuclear chemistry , particle size , materials science , klebsiella pneumoniae , antibacterial activity , chemistry , crystallography , chromatography , bacteria , composite material , crystal structure , biology , biochemistry , genetics , escherichia coli , gene
ZnO-Ag nanocomposite synthesis has been successfully carried out by ultrasonication using clove oil as a reducing agent. Synthesis reactions were carried out in 30, 60, 90, 120, 240, and 300 minutes time variations. ZnO-Ag crystallinity was analyzed using X-Ray Diffractometer (XRD) and the average particle size was calculated using Scherrer equation. The morphology of ZnO-Ag which has the smallest particle size was analyzed by Scanning Electron Microscope -Energy dispersive X-Ray Spectroscopy (SEM-EDX). ZnO-Ag antibiofilm activity against Klebsiella pneumoniae was determined by microtiter plate test. Based on research that has been done, the best time for synthesizing ZnO-Ag with clove oil through ultrasonication is 90 minutes, the resulting particle size of 21.46 nm with the composition of 51.4% Ag (cubic), 20% wulfingite (orthorhombic), 22.9% Zn(OH) 2 (orthorhombic), and 5.7% ZnO (cubic). ZnO-Ag has strong antibacterial activity against K. pneumoniae and can inhibit the growth of K. pneumoniae biofilms.