Open AccessThe influence of Ag concentration on the antibacterial properties of plastic made from silica immobilized with EDTA-Ag combined with chitosan
Author(s) -
Hermania Em Wogo,
Marini T. Tanuab,
Luther Kadang,
Antonius R. B. Ola
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/823/1/012034
Subject(s) - chitosan , antibacterial activity , staphylococcus aureus , escherichia coli , nuclear chemistry , chemistry , antibacterial agent , materials science , chromatography , bacteria , organic chemistry , antibiotics , biochemistry , biology , genetics , gene
Antibacterial plastic is made by using composites silica gel immobilized EDTA-Ag combined with chitosan. Silica gel was formulated with three variations of Ag concentrations (i.e., 10 -3 , 10 -4 , and 10 -5 M) and then tested for its antibacterial activity against Escherichia coli and Staphylococcus aureus . Result of the antibacterial test showed that the immobilized EDTA-Ag silica gel product could inhibit the growth of Staphylococcus aureus and Escherichia coli with the diameter of zone inhibition around 15.9 and 15.6 mm respectively at the lowest Ag concentration of 10 -5 M. This result showed that higher concentration of Ag led to the decrease of antibacterial activity. The obtained products were then synthesized into plastic with variations of chitosan weight of 0.3 and 0.7 grams. The antibacterial test confirmed that the plastic had strong antibacterial properties with the diameter of zone inhibition for Staphylococcus aureus and Escherichia coli was 16.7 and 15.9 mm, respectively. The percentage of degradation was observed at 6.02 to 63.17% while water absorption test for plastic with 0.3 gram chitosan was 86.51% and for plastic with 0.7 gram chitosan was 16%. This indicated that more chitosan additions were able to expand antibacterial properties and reduce the absorption of plastic against water.