Open AccessStudy on Separation and Purification the Secondary Metabolites of Oudemansiella Mucida
Author(s) -
Xu Deng,
Risheng Li,
Shupei Deng
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/782/2/022008
Subject(s) - sephadex , column chromatography , chemistry , chromatography , silica gel , ethyl acetate , carbon 13 nmr , dept , pyrazine , organic chemistry , stereochemistry , enzyme
Oscillation culture was carried out on Oudemansiella mucida. The mycelia were extracted with ethyl acetate, separated by silica column chromatography and Sephadex LH-20 dextran gel chromatography. Though determination of chemical and chemical properties of chemical constituents, characterization by organic spectra (MS, 1H NMR, 13C NMR, and DEPT), and comparison with standard compounds, the three secondary metabolites from mycelia were finally identified to be ergosta-4, 6, 8(14), 22-tetraen-3-one; 5α, 8α-epidioxy-(22E, 24R)-ergosta-6, 22-dien-3β-ol, Hexahydropyrrolo [1, 2-α] pyrazine-1,4-dione.