Open AccessBioethanol production from microalgae Chlorella sorokiniana via simultaneous saccharification and fermentation
Author(s) -
N. J. Tatel,
Cynthia F. Madrazo
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/778/1/012039
Subject(s) - chlorella sorokiniana , biofuel , fermentation , biomass (ecology) , ethanol fuel , cellulase , hydrolysis , food science , chemistry , raw material , enzymatic hydrolysis , pulp and paper industry , ethanol fermentation , bioprocess , central composite design , chlorella , botany , microbiology and biotechnology , response surface methodology , chromatography , biochemistry , biology , agronomy , algae , paleontology , organic chemistry , engineering
This study aimed to investigate the performance of a microalgal feedstock Chlorella sorokiniana in bioethanol production through simultaneous saccharification and fermentation (SSF). The major limiting factor of SSF is the compensation between optimum hydrolysis and fermentation parameters. Using a central composite design (CCD), three parameters were optimized: biomass concentration, temperature, and pH. For each run, the enzyme mixture (cellulase and amylase) was fixed at 25 g/L, fermentation time at 72 hrs, and inoculum size of Saccharomyces cerevisiae at 20% (v/v). The freeze-dried biomass is simultaneously mixed with the enzyme mixture and the inoculum and placed inside an incubator shaker. The microalgal biomass is found to contain 58.78% total reducing sugars (TRS). Highest ethanol yield obtained is 0.504 g ethanol/g glucose. Using CCD helps navigate the design space and bioprocess.