Open AccessThe Numerical Estimation of Mass Transfer Coefficient of Oxygen in the Large-Scale Suspension Culture of iPS Cells
Author(s) -
Ryosuke Isobe,
Yoshiki Kanemaru,
Atsushi Sekimoto,
Yasunori Okano,
Masahiro Kinooka
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/778/1/012030
Subject(s) - mass transfer , mass transfer coefficient , suspension (topology) , turbulence , sedimentation , oxygen , mass culture , chemistry , sedimentation coefficient , limiting oxygen concentration , induced pluripotent stem cell , mechanics , materials science , physics , chromatography , mathematics , biology , biochemistry , anthropology , sociology , homotopy , organic chemistry , pure mathematics , enzyme , paleontology , embryonic stem cell , sediment , gene
In order to practically apply induced pluripotent stem (iPS) cells to regenerative medicine, a large amount of undifferentiated iPS cells should be produced by using an automated/scaled-up suspension culture system. However, in large-scale culture, oxygen supply to iPS cells away from the gas-liquid interface can be insufficient. In this numerical study, the oxygen supply performance is quantitatively evaluated by estimating the volumetric mass transfer coefficient of oxygen in the suspension culture of iPS cells. And, focusing on shaking culture, where shear stress that causes death or differentiation of iPS cells is relatively reduced, we compare two different shaking methods: one direction rotation (ODR) and periodic alternate rotation (PAR). The validity of the volumetric mass transfer coefficient calculation is confirmed by comparison with the experiment. The PAR method is superior to the ODR method in terms of oxygen supply because of higher turbulence intensities, but it is much less energy efficient than the ODR method. In the ODR method, the cell size is thought to be non-uniform since iPS cells are aggregated due to cell sedimentation. On the other hand, in the PAR method, cell sedimentation is suppressed by controlling the Froude number ( Fr ).