Open AccessDetermination of Arctiin in Fructus Arctii by Capillary Electrophoresis
Author(s) -
Xiaohan Zhou,
Haixing Liu,
Lintong Wang
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/772/1/012032
Subject(s) - capillary electrophoresis , chromatography , borax , chemistry , content determination , methanol , traditional medicine , high performance liquid chromatography , medicine , organic chemistry , raw material
This paper investigated the determination of arctiin content in Fructus Arctii by high performance capillary electrophoresis (HPCE) method. The borax solution of 37.5 mmol concentration containing 12.5% methanol was chosen as buffer solution. The experiment was performed at a constant voltage of 16 kV and UV detection wavelength of 277 nm. The content of arctiin in Fructus Arctii was 26.45mg/g (RSD=3.34%) (n=7). The recovery was in the range of 87.6%-111.8% (n=5). This method is suitable for the detection of the content of arctiin in Fructus Arctii.