Open AccessA new Cghd I gene with negative regulation on the synthesis of chaetoglobosin A
Author(s) -
Cheng Jiang,
Jinzhu Song,
Junzheng Zhang
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/768/5/052049
Subject(s) - chaetomium globosum , gene , genetics , biology , strain (injury) , dna , genome , yield (engineering) , agrobacterium , computational biology , transformation (genetics) , botany , anatomy , materials science , metallurgy
Chaetoglobosins have wide application prospect, but the low yields make it not widely used. The aim of this study was to obtain high-yielding strains and to find the genes regulating the synthesis of chaetoglobosins. A strain with yield increased to 115% was obtained through inserting a short-DNA randomly into the genome of Chaetomium globosum by agrobacterium. At the same time, inverse PCR was used to determine the insertion position of T-DNA. A new gene Cghd I with negative regulation on the synthesis of chaetoglobosin A was found, indicating that the detectable random insertability mutation technique established in this paper can be used to obtain high yield strains and find regulatory genes.