Open AccessEvaluation of dissolution of purified bromelain from pineapple core extract (Ananas comosus [L.] Merr) in the form of chitosan-coated microspheres
Author(s) -
T K Zahrah,
Siswati Setiasih,
Sumi Hudiyono
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/763/1/012032
Subject(s) - bromelain , glutaraldehyde , ananas , chitosan , microsphere , chemistry , chromatography , dissolution , nuclear chemistry , enzyme , biochemistry , papain , chemical engineering , organic chemistry , botany , biology , engineering
The aim of this study is to coat the bromelain from pineapple’s core into chitosan microspheres crosslinked by glutaraldehyde to maintain its activity until it reaches the intestines. The crude enzyme was first purified by ammonium sulfate precipitation method, followed by the dialysis. The bromelain fraction of the dialysis has a specific activity of 294.44 U/mg with a purity level of 15.68 fold compared to the crude enzyme. Coating bromelain using glutaraldehyde crosslinked microsphere showed an efficiency of 87.14 %. The dissolution test results showed the release rate of bromelain in artificial gastric fluid is 9.35 % and in artificial intestinal environment is 79.92 %. The result of this study indicates that chitosan-coated microspheres are good enough as bromelain coatings for slow-release matrices.