Open AccessCallus-induced explants selection of ‘Pyrus betulifolia’
Author(s) -
Yufei Gao,
Jiufeng Chai,
Ying Dong,
Lingling Kong,
Yong Zhang
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/740/1/012196
Subject(s) - callus , explant culture , hypocotyl , biology , botany , pear , regeneration (biology) , transformation (genetics) , in vitro , horticulture , microbiology and biotechnology , genetics , gene
Efficient and stable in vitro regeneration system is the basic condition and key factor for the genetic transformation of pear. In order to find the most suitable explants to induce callus, three different explants, including seeds, hypocotyls and young leaves, were selected to induce callus respectively, and the regeneration ability of the three explants was compared, and finally the best material was selected. The results showed that loose and plump callus could be induced by using hypocotyl as explant in a short time.