Open AccessBrowning Treatment in Tissue Culture of ‘Miliang 1’ Delicious Kiwifruit
Author(s) -
Wang Rui,
Qingling Song,
Lingling Kongd,
Yong Zhang
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/740/1/012194
Subject(s) - browning , explant culture , callus , tissue culture , horticulture , food science , chemistry , biology , in vitro , biochemistry
Explant browning is a common problem in tissue culture and has become a limiting factor in the development of kiwifruit tissue culture. The effects of different sampling time and disinfection conditions on tissue culture browning of ‘Miliang 1’ delicious kiwifruit stem segments were studied in this study. Finally, the best months of harvest and the best disinfection conditions were screened out. The results showed that the browning rate of ‘Miliang 1’ taken in April was significantly lower than other time periods, and the browning rate gradually increased with time, reaching a maximum of 33.3% in September, significantly higher than other times. There was no significant difference in the browning rate among May, June, July and August. When the disinfection treatment was 75% alcohol 10s + 0.1% mercury liter for 2 min, the number of pollution was much higher than other treatments, the disinfection treatment time was obviously insufficient. Whatmore, the longer the disinfection time, the less the number of pollution, but the more the number of browning was increased. When the disinfection treatment was 75% alcohol 30s+0.1% mercury 5min, the number of browning was significantly higher than other treatments. With the increase of alcohol and mercury treatment time, the number of explant contamination decreased, but the number of death increased significantly. Generally speaking, the final survival rate of callus induction in the treatment of 75% alcohol 20s + 0.1% mercury for 5min was significantly higher than other treatments, which was more suitable for the callus induction culture and disinfection of ‘Miliang 1’ kiwifruit.