Open AccessImmobilization of Alcohol Dehydrogenase in membrane: Fouling mechanism at different enzyme concentration
Author(s) -
Farazatul Harnani Ismail,
Fauziah Marpani,
Nik Raikhan Nik Him
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/736/5/052024
Subject(s) - membrane fouling , chemistry , membrane , enzyme , alcohol dehydrogenase , fouling , chromatography , dehydrogenase , enzyme assay , chemical engineering , biochemistry , engineering
Fouling induced enzyme immobilization on polymer membrane with controlled variables can retain enzyme stability and performance. In this study, the effect of enzyme concentration towards the fouling-induced enzyme immobilization was investigated. Alcohol dehydrogenase (ADH) was used as model enzyme to be immobilized on poly(ether) sulfone (PES) membrane at different concentration (0.05, 0.10 and 0.20 g/L). The transmembrane pressure and pH were fixed at 2 bar and pH 7 respectively during the process takes place. The result shows that higher enzyme concentration gives higher loading efficiency with 83.95%. Hermia’s model predicted that standard and intermediate blocking dominating the fouling mechanism at all enzyme concentration tested.