Open AccessA novel enhancement for the start-up of methane fermentation reactor by inoculating the acclimated sludge as a seeding material
Author(s) -
Nga Thi Dinh
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/736/4/042041
Subject(s) - glycerol , anaerobic exercise , methane , fermentation , substrate (aquarium) , pulp and paper industry , start up , inoculation , microorganism , chemistry , food science , biology , waste management , zoology , bacteria , horticulture , biochemistry , ecology , organic chemistry , physiology , business administration , genetics , engineering , business
This study aimed to evaluate the strategy for accelerating the start-up of anaerobic reactor treating glycerol. The experiment was carried out for 25 days duration in two cases: (1) gradually increase of glycerol content in substrate from 33% to 67% to 100% after 13 days (runs: G1 and B1); (2) feeding with 100% glycerol content in substrate from the start (runs: G2 and B2). G1 and G2 were inoculated by one third of acclimated granular sludge to treat glycerol and two third of granular sludge taken from a brewery wastewater treatment system. In results, the average methane production rate (L CH 4 L −1 day −1 ) of runs G1; B1; G2; B2 was 0.56; 0.39; 0.42; and 0.14, respectively. G1 was the best performance among runs. G2 and B1 were unstable during the start-up. B2 was fluctuant throughout the duration time. The methane production rate of G1 and B1 was highly correlated and DNA concentration of Genus Trichococcus in the sludge. This study suggests that gradual feeding substrate during start-up could provide a stable performance compare to the shock feeding. Acclimated sludge which contained functional microorganisms of treating glycerol could accelerate the start-up of new anaerobic digester.