Open AccessOptimization of reaction conditions for the production of cyclodextrin (CD) using cyclodextrin glucanotransferase (CGTase) immobilized on hollow fiber membrane
Author(s) -
Syahida Suhaimi,
Rohaida Che Man,
N. Jamil,
Zatul Iffah Mohd Arshad,
Shalyda Md Shaarani,
Siti Kholijah Abdul Mudalip,
Safwan Sulaiman,
Aizi Nor Mazila Ramli
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/736/4/042005
Subject(s) - cyclodextrin , chemistry , response surface methodology , adsorption , hollow fiber membrane , starch , fiber , membrane , chromatography , chemical engineering , biochemistry , organic chemistry , engineering
Cyclodextrin glucanotransferase (CGTase) is an enzyme that convert starch into cyclodextrin (CD) by transglycosylation reaction. The CD has been used in various industries due to the unique characteristics. However, the production of CD is usually restricted due to the instability of the enzyme which easily denatured during the reaction process. Thus, enzyme immobilization technique was applied and optimization of reaction conditions was conducted to enhance the amount of CD production. In this study, CGTase was immobilized on polyvinylidiene difluoride (PVDF) hollow fiber membrane by adsorption. The optimization of the reaction conditions using response surface methodology (RSM) on the production of CD was studied. Under optimized conditions (2.8% w/v of soluble potato starch concentration, 45.2°C of reaction temperature and pH 5.6), the production of CD was 5.65 mg/mL, about 2-fold compared to the value before optimization process. Therefore, the immobilized CGTase on hollow fiber membrane proved to be valuable for the enhancement of CD production.