Open AccessExtraction optimization for antioxidant phenolic compounds in black grass jelly (Mesona palustris BL) using response surface methodology
Author(s) -
H Hendratama,
Kun Harismah,
Ariza Fuadi
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/722/1/012019
Subject(s) - phenols , chemistry , phenol , antioxidant , response surface methodology , tannin , proanthocyanidin , solvent , extraction (chemistry) , polyphenol , chromatography , food science , reagent , organic chemistry
Black grass jelly is a traditional plant that is more widely used as medicine to prevent carcinogenesis, diabetes, and hypertension, to overcome diarhea, because it is antimutanogenetics, stabilizers, and immunomudulators, besides black grass jelly also contains antioxidants. This study examines changes of temperature and solvent ratio toward the composition of phenols, tannins, and flavonoids which are antioxidant constituents. Analysis of phenols and tannins uses folin quality reagents, while analysis of flavonoids uses the spectrophotometer method. The change of temperature and solvent ratio gives effect to change of phenol, tannins, and flavonoids amount. Optimal extraction of phenol, tannin and flavonoids using the response surface methodology (RSM) occur at a temperature range of 94-100°C with a solven ratio of 23-24 ml/g.